DNA
Part:BBa_K323153
Designed by: Tina Ilc Group: iGEM10_Slovenia (2010-10-25)
Scrambled DNA program
This part contains a DNA program - a specifically designed nucleotide sequence containing 6 zinc finger binding sites. The program was designed in such a way, that chimeric enzymes (fused with zinc finger domains) for synthesis of violacein bind to the sequence in the order required to produce the final product. The part contains binding sites for zinc finger proteins in the following order: Zif268 - PBSII - Jazz - Blues - HivC - Gli1.
We have bound five chimeric enzymes, encoded by Part:BBa_K323135 and Part:BBa_K323132 to the program to show that a correct arrangement of enzymes on the DNA program is important for the progress of the reaction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |